Cell Phones and Driving free essay sample

A glance at the threats of mobile phones and cutting edge innovation. This paper examines why PDAs are so hazardous by taking a gander at an assortment of mishaps and wounds brought about by mobile phones. The creator contends for the need of national enactment forbidding hand held phones while driving. Incorporates an enticing conversation of the way that trend setting innovation and correspondence has influenced society. Radios, pets, others in your vehicles. What do these things share for all intents and purpose? They are generally interruptions to an individual that is driving a vehicle. Anyway there is one other interruption that is more awful than any of these consolidated. This interruption can make you multiple times as liable to crash. It tends to be similarly as unsafe as tanked driving (Frankfield 1). What is this interruption? It is a straightforward gadget that heaps of individuals in our nation own and utilize day by day. It is a gadget that can unexpectedly spare our lives in the midst of peril. We will compose a custom article test on Mobile phones and Driving or on the other hand any comparable point explicitly for you Don't WasteYour Time Recruit WRITER Just 13.90/page It is a mobile phone. It is therefore that enactment must be passed broadly forbidding the utilization of hand held PDAs while driving.

Analyzing the key elements of the nature-nurture controversy Essay

Examining the key components of the nature-sustain debate - Essay Example These elements include different measurements including physical situations, for example, pre-birth sustenance, social conditions, for example, the media among others. The nature versus support banter is a contention worried about the intrinsic characteristics of an individual versus the individual encounters used to decide or make contrasts in people, in their social and physical qualities. Mentally, nativism has the suggestion that a few aptitudes or capacities are normally hard wired in a person’s cerebrum at the hour of birth. Then again, Empiricism or behaviorism suggests that hypothetical information that state completely that an individual’s information can just come through tangible experience. In such manner, certain logical specialists have demonstrated that youngsters become the result of the conditions that they live in, or they obtain information to do the things they do by sustaining. Others contend that, individuals act in specific courses through genetic and this establish to nature. Most scholars are additionally in an understanding that, nature and sustain are both interwoven, and they have an impact in most human viewpoints, for example, feeling, comprehension among others. The inquiries behind the human advancement just as the elements that impact it have taken focal significance in brain research since the time the start of the order. The contention that human advancement includes these two variables has been contended for quite a while by the two scholars and therapists. Progressive thoughts that came in the nineteenth century and their changes, for example, industrialization, Darwinism, and the cancelation of subjection made an induction to the clashing understandings that existed about the world and human life. In fixing the impacts of condition and qualities to individual, conduct geneticists for the most part play out the twin and appropriation considers. Such examinations target decaying the fluctuation that exists in a populace into natural and hereditary parts. By the utilization of the examination of the connection in an excellent measurement like the insight test (IQ) scores in indistinguishable twins and the congenial twins, it feasible for analysts to hypothetically work out the near impacts of both nature and support on this specific measurement. Sandra Scarr, for instance, revealed a connection for the IQ test scores for indistinguishable twins of 0.86 and for the friendly twins of 0.55 she demonstrated that, indistinguishable twins had scores that are near each other when contrasted with those of the brotherly twins. This means that genetic. Opposite, where the IQ scores are 100 percent decided hereditarily, the indistinguishable twins’ connection would be 1.00 as indicated by this model, heredity appears to have a noteworthy job. This doesn't imply that it is surely the determinants in IQ tests. Specialists additionally screen concordance rates, notwithstanding the heritability gau ges. Concordance rates are the ones at which the improvement of a trademark happens in the two twins (Novel guide, 2011). In this investigation, the need or accessibility of a particular state of mind is generally considered being a phenomenal model. Under circumstances that the two twins demonstrated some clinical misery in all matches that were analyzed in the examination, this would give a concordance pace of 100 percent in such a case. Opposite, if, in each pair, there was one person who had the clinical discourage

Being productive over break

Being productive over break This post is delayed a few days due to publishing, but still relevant. Welcome back, everyone! I have to say, its great to be back, even though life is a bit busy! Part of what is helping me stay calm is the fact that I did a bit of work over break. First, let me acknowledge the fact that it can be hard to be productive over break. However, it certainly is possible, and youll thank yourself for your somewhat simplified life after break. If not everything is do on Monday, you dont absolutely have to do everything. Just begin doing something, and try to make as much progress as you can. Seriously, even just an hour of work a few days over break will help. Doing a little something over break will boost your confidence when you get back. Over break, I did some paperwork and half a project. Not too much, but it has made this week easier to handle! Sarah Class of 2018 I'm from Grand Rapids, Michigan. I'm majoring in Communication in the College of Liberal Arts and Sciences.

A Radical Islamic Group in Iraqi Kurdistan Ansar al-Islam...

Introduction Ansar al-Islam (AAI) issue titled the magazine and TV in September 2001, when it killed forty-two Patriotic Union of Kurdistan (PUK) fighters. This was awakened up the Kurds, who rapidly built up a conventional defensive front. It was very clearly apparent that the Kurdish was the main objective as priority of the new jihadist war, (Schanzer 2004: 43). The group was established in Kurdistan region in 2001 as a Salafist Islamist organization imposed a strict application of the Sharia in some villages around Halabja city, near the Iranian border. After the US invasion of Iraq, AAI became a clandestine insurgent group, which fought against the Kurdish political parties and the US armies and its Iraqi allies. Currently, the group†¦show more content†¦While Kurdish had unsuccessfully contested parliamentary elections in 1992, the Islamic Movement of Kurdistan kept it away from participation Kurdish administration, relying instead on growing and strengthening a distinct administra tive, military and political structure in fields under its own power, (Zenko 2009: 618 Human Right Watch 2003). The IMK fragmented over power struggle as well as policy distinctions during 1997. Some smaller groups with the IMK, which advocated a more radical and ultra-orthodox Islamic ideology, also splintered form, the movement at different times. Of these groups, the most significantly militaries were factions namely the Soran Forces, Tawhid and Hamas. These smaller splinter groups themselves regularly unified, On September 1, 2001, they announced Jundal Islam (Soldiers of Islam) organization. The group very soon changed its name for Ansar al-Islam (Supporters of Islam) in December 2001, and then it declared jihad (holy war) against apostates and other secular political parties in Kurdistan, that was because the group did not accept the Kurdish sculler administration. AAI was financially and military sponsored by al-Qaeda, Iran and Wahhabi basic in Saudi Arabia. Najmuddin Faraj Ahmad (Mala Fateh Krekar), whom a long member of the IMK became the leader (Emir) of Ansar al-Islam, (Joscelyn Ro ggio 2012, Zenko 2009: 618-19 and Rubin 2004: 6). However, in the face of rise

Aging Bias For The Elderly - 1446 Words

It is important to understand that patients have been experiencing aging bias for a very long time. It is also evident in the medical profession as well, and one needs to do more to ensure that everyone is treated fairly especially the elderly patients. The elderly patients need to treat with the utmost respect and dignity. One needs to understand that the elderly are the ones who pave the way for the younger generation. Therefore, it is important that one takes the time out of their busy assignments to listen to what the older patients have to say and respond to them in an appropriate manner. This paper will discuss how aging has impact one nursing practice, aging bias that one has witnessed during their nursing practice, and a community plan to discuss aging bias. Aging Bias It is important to note that whenever one comes across an elderly person, whether it would be working or on the train. It is important to always make it one’s point of duty to ensure that the elderly is comfortable. If the elderly person is standing and one is sitting, it would be the right thing to do by offering the seat to the individual, this also goes for nursing as well. Whenever one is working with an elderly person, it is important that one treat them like their grandparents, that is, with the utmost respect and dignity that they deserve. Also, if they are from a different culture or background, one would always try to learn something new from that culture. It is important to treat everyone theShow MoreRelatedIs Ageism The Intentional And Subconscious Discrimination Against Older Adults? Essay1265 Words   |  6 Pagesyounger generation does not understand that and dismisses the elderly. Showing respect and dignity to the older adult (OA) should be a courtesy ingrain ed in each of us, sadly, that has been forgotten in today’s society. The younger generations must learn the importance of respecting and take the time to listen to what the OA has to say and spend quality time with them. Children should be taught from an early age to treat the elderly with respect and dignity, and it should be carried with them throughoutRead MoreGender, Discrimination, Bias, And The Hostility Against Older People942 Words   |  4 Pagesdiscrimination, bias, and the enmity against older people. A great example of ageism is rejecting an older job applicants in favor of a younger one. Three theory apply to aging the elderly. Our first theory is called structural-functional theory. As the author of our textbook, John Macionis, agrees that this approach views society as a complex, but interconnected system, where each part works together as a functional whole (p.16). The author of the book applies this approach to the aging topic and showRead MoreThe Dangers Of The Elderly1582 Words   |  7 Pagesyears. The effect of the elderly living longer is that the occurrence of illness and disabilities, as well as recovery time, is increased (Lovell, 2006). Associated with this is the increase in demand for health care resources, and the negative stereotype of the elderly being an economic burden (Lovell, 2006). The stereotype of the elderly being frail is perpetuated by the media’s promotion of youth and vitality, as well as the biased reporting of the negative impacts of aging (Lovell, 2006). HoweverRead MoreReflection Paper On Aging1710 Words   |  7 Pagesthe e mbodied aging and experience by creating a storyline based on the character, Billy, shared the good news of him finally proposing to his much younger girlfriend with his dearest friends and wanted them to be supportive in his decision-making. All four friends decided to escape from their daily lives a couple of days in order to throw a bachelor party together for Billy in Las Vegas (Last Vegas, 2013). They have been best friends since childhood, yet have experienced a different aging journey (LastRead MoreThe Treatment Of Muscle Mass Essay1569 Words   |  7 Pagesweight. Their function is to provide the body movement, strength, respiration, balance, posture and to regulate the temperature (Lenk et al., 2010). Sarcopenia has since been defined as the loss of skeletal muscle mass and strength that occurs with aging. ( Cruz-Jentoft et al., 2010). Sarcopenia is considered one of the geriatric syndromes because its prevalence in older populations (Chien, et al 2008). Sarcopenia leads to functional disabilities, decrease mobility, falls, and fractures, which leadRead MorePico : Depression : Pico1296 Words   |  6 Pagesanalyzed to form the answer to the Pico question. The purpose of this paper is to formulate a question and research on the topic of depression in the elderly, using the PICO format. Part 1: Pico Analyses of Research Topic Depression is lack of enjoyment and a general feeling of sadness (Brouwer-Brolsma,et al., 2015). It can happen to all ages, but, the elderly are, especially prone (Brouwer-Brolsma,et al., 2015). It is often misdiagnosed, because many of the symptoms such as, irritability, sleep disturbancesRead MoreThe Population Of The United States900 Words   |  4 Pages With the continual increase in the elder population, whether or not an aging population is beneficial to society has become widely debatable. While many believe that this top-heavy type of population distribution poses a colossal obstacle in terms of economic development in the long run, others maintain that it is a testament to the development of a society. Given that a high proportion of elderly citizens puts immense pressure on the working force and would be a burden on the healthcareRead MoreThe Face Of The Hiv Epidemic Essay1498 Words   |  6 Pages The face of the HIV epidemic has changed. People ages 50 and older now represent the fastest growing segment of HIV positive adults in the United States (Sankar et al, 2011). A workshop on HIV Infection and Aging estimated that by 2015, adults aged 50 and older will make up approximately 50% of all HIV/AIDS in the U.S. (Effros et al, 2008). Factors, including decreased efficiency of the immune system and decreased likelihood that older adults have been tested for HIV, increase the vulnerabilityRead MoreThe Effects Of Ageism On The Delivery Of Nursing Care For The Older Person1736 Words   |  7 Pagesdiscrimination of any age group, however for this essay, it will be used in the context of discrimination of the elderly population. The elderly population accounts for 50% of hospital stays, 70% of home health services and 90% of residents in aged care homes where a further 90% of residents fall victim to and experience ageism throughout their stay (Fitzpatrick Kazer, 2012). The eld erly account for such a large proportion of the population using healthcare services, therefore, in turn, is the reasonRead MoreWorking With The Elderly Population1475 Words   |  6 Pagesyears in my life I knew that I had a passion to care for the elderly (aging) population. From this passion, it ignited a fire so strong that I pursue my certified nursing assistant certification to gain a better understanding of working with the elderly population. By doing so, it has been a great benefit to my life; aging is an inevitable experience that we all will go through during our life. It is important to ensure that our elderly individuals are still able to enjoy the best quality of life

Business proposal for Papadeaux Free Essays

Pudenda is a successful upscale casual restaurant concept with a loyal following. But no restaurant is immune to the highly competitive climate of the industry. Recent studies have shown the upscale casual segment losing market share to both fast and traditional casual restaurants. We will write a custom essay sample on Business proposal for Papadeaux or any similar topic only for you Order Now They also show that consumers find value in upscale casual dining by weighing the food quality and the overall experience against the relatively higher price (Techno, Inc. 2013). We analyzed customer reviews to discern what Pederasty’s guests are responding to In terms of value. Based on this analysis, customers respond overwhelmingly positively to food quality, and food quality Is the most critical factor In their value calculation. Price Is still an issue, even those delighted by food quality comment on high prices. Customer comments also suggest that portion sizes may be overly large, and that the investment in large portions is out of step with its importance to the guests. Pudenda may benefit from featuring new menu items with reduced portion and price. Such offerings at other restaurants have led to increased traffic, revenue, and per person average (APP) (Jennings 2008). . Industry background Casual dining has grown over the last thirty years along with tremendous growth in American’s restaurant spending. Half of every food dollar In this country is spent in restaurants, which Is double the spending level In the ass’s (Pocket Fastback, 2013). The recession has made a competitive Industry even more competitive. A recent Techno survey on casual dining revealed a three tiered system divided Into fast casual, traditional casual, and upscale casual, with consumers trading up and down between these levels based on their needs and perceptions of value. 5% of those surveyed reported visiting a fast casual restaurant in the past month, 80% had visited a traditional casual restaurant, and 40% reported eating at an upscale casual restaurant. 41% of those surveyed reported finding value in upscale casual when weighing food quality and overall experience against the relatively higher price (Techno, Inc. 2013). The issue for operators in upscale casual is getting consumers to trade up, and to trade up more frequently. 3. Pudenda Seafood Kitchen Pappas Restaurants began In 1 966, when brothers Chris and Harris Pappas opened Dot’s Coffee Shop In Houston. Today they operate over eighty restaurants across an array of concepts Including BBC, Tex-Meg, and fine dining. All Pappas restaurants are guided by the Standards of Excellence: Providing superior quality food, providing Kitchen, founded in 1986, is an upscale casual dining restaurant featuring gulf coast seafood and Cajun dishes. It has become the company’s largest concept, and is set to absorb Pappas Seafood House, the company’s first upscale casual seafood concept, growing its size in the Houston area by five stores. 4. Review Analysis 4. 1 . Methodology One of the most influential ways information is transmitted is word-of-mouth (Manikin Goods, 2002). Word-of-month has an effect on not only potential customers, but also the satisfaction level of current guests. One of the most powerful methods to generate word-of-mouth is online customer review (Dollars, 2003). A total of 441 postings from Yelp. Mom made between September 2012 and September 2013 for all Pudenda locations, were reviewed for this analysis. Chin et al. (2004) found that recommendations have a positive relationship with sales, while consumer ratings were shown to be unrelated. Therefore our analysis focused on content rather than ratings. Content was divided into four main categories: food, service, value and environment/atmosph ere. Each of these was further divided into subcategories, which were then coded positive or negative based on comment content. 4. 2. Food Food was the most commented on of the major categories. As displayed in Figure 2, the response to food quality was overwhelmingly positive. Comments such as, â€Å"the cocktail shrimp is well seasoned and super fresh,† â€Å"Just about everything on the menu is delicious,† and â€Å"the crayfish fondue is amazing as well as everything on the menu,† indicate that the customers are not only satisfied but also highly delighted. There were more positive comments on food than comments in any of the other categories. 4. 3. Service Restaurants always receive both positive and negative comments and Pudenda is not an exception. A calculation of the number of positive and negative comments on service, however, shows customers are mostly satisfied by the service. Over 75 percent of comments are positive (see Figure 3). Based on the graph and actual comments, we concluded that the gap between expectation and service delivery is mall in Pudenda case. 4. 4. Environment When commenting on environment and atmosphere, guests tended to be less specific when they were positive. Negative comments focused on noise level, crowding complaints, and wait time. The number of these complaints is relatively high compared with the number of positive comments (see Figure 4). This suggests that the atmosphere of Pudenda is more crowded and noisy than guests expect. 4. 5. Value determined by factors such as price and food quality. As seen in Figure 5, roughly half of all comments in this category stated that Pudenda is expensive. Only 37% recent of comments relate that the price is worth the overall experience, however, the percentage of comments stating that the experience is â€Å"not worth it† is much lower. While the latter two categories are mutually exclusive, significant overlap exists between both and the former category. This suggests that while guests’ subjective view of value is affected by the service delivery, their objective view of price is left unaltered. 5. SOOT Analysis 5. 1 . Strengths Pederasty’s greatest strength is consistently providing high quality food and service. Doing so is a stated goal of the company, and guest reviews confirm that Pudenda succeeds in following through on that goal. This achievement has garnered the concept a strong reputation. Many reviewers mentioned being repeat customers, or called the experience, â€Å"excellent as always. † This again speaks for the consistency many strive for in this industry, but few are able to attain. 5. 2. Weaknesses Pederasty’s main competitive weakness is it’s comparatively high prices. Providing high quality food requires an investment in quality products and labor. Seafood is often associated with high costs, because freshness is key to its quality. Additionally, he review analysis found complaints about the atmosphere. Its prices are more similar to lower end fine dining restaurants than its casual dining peers. Pudenda’ more family friendly atmosphere may be at odds with what some expect from the environment when they look at the menu prices. 5. 3. Opportunities Despite high prices, Pudenda receives higher average ratings than its competitors. Its greatest opportunity lies in leveraging its high quality against competitors. Strategies that focus on attracting new customers to trade up from lower priced competitors so that they can experience the difference in quality may rove successful. Based on the high rating and positive comments from the Albuquerque and Phoenix locations, further expansion in the Southwest may be worthwhile. 5. 4. Threats Pederasty’s faces threats from lower priced competitors. High price is a major component leading consumers to dine at upscale casual restaurants less frequently. Large competitors in this group, like Red Lobster, use nationwide promotions offering greater value to increase traffic at their stores. More similarly priced restaurants tend to have a different market focus, and provide a more elegant and less family oriented atmosphere. Guest Perception of Value Figure 6 GAP Analysis A GAP analysis revealed that there was little mismatch between guest expectations and perceptions. The only area for concern was the atmosphere. As stated, complaints in this category were relatively high in proportion to positive comments, suggested the atmosphere is louder and more crowded than many guests expect. Relation to value based commen ts than any type of complaint about atmosphere. Further, price is important factor driving consumers to trade down in the casual dining spectrum. As recommendations are related to sales, comments about price would be taken seriously. While reviews indicate that guests are satisfied, these may not be enough. Oh (2000) found that customers’ perceived value has a greater effect on their intention to return, and, more importantly has a greater effect on their long term patronage. In order to further analyze guests’ perception of value, we returned to online customer reviews. This time, we focused our research on the Houston area locations. Houston was selected because it has more locations than any other area, and, as the place of its founding, the area has the greatest familiarity with both Pudenda and the Pappas brand. We analyzed reviews from both Yelp. Com and Trip Advisor from the same time period, September 2012 through September 2013, a total of 490. Two methods were employed. First, the same coding method as before was used to compare against the early data in order to check for differences. Second, quotes relating to value were pulled from the reviews in order to better understand the range of guest attitudes and determine possible trends. The data from the content analysis was similar to what we saw previously. Roughly half of all value related comments referenced high prices. No new issues turned up in any of the other disagrees, so we proceeded to review quotes pulled from the article. 6. 1 . General Perceptions Guests tended to have a favorable impression of value while also acknowledging the high price, for example, â€Å"Pudenda is a little pricey but very good,† â€Å"The price is not low but it is worth the price for good food and service,† and â€Å"The prices are high but the food is excellent. † These responses are in line with the results from the Techno survey (2013) discussed earlier. It is important to note that over 70% of these quotes specifically mentioned food quality and linked it with a positive impression of value. 6. 2. Price and Negative Behavioral Intention Guests with a negative impression of value tended to express their view in terms of price. Quotes such as, â€Å"Overpriced but okay I guess†¦ â€Å", â€Å"†¦ Over priced mediocre food†, and â€Å"†¦ A little overpriced for what you get,† were typical of this group. In addition, negative comments about price were frequently linked with a negative behavioral intention to return. Most interesting was a subgroup of almost 30%, who specifically expressed that while satisfied, they would return less frequently due to price. Typical quotes in this range included, â€Å"I’ll be back but Rajah Cajun is definitely more affordable and so will be a more frequent stop for me! † and â€Å"The prices have increased steadily and I don’t go as often†¦ â€Å". These quotes are representative of consumers who find value in the quality of the experience, and do trade up to upscale casual, but do so less frequently based solely on price. . 3. Food Quality as the Most Critical Factor Whether guests express a positive or negative perception of value they most frequently link value with their perception of food quality. 70% of positive value impression quotes mentioned food quality, as did 44% of the negative value laity (â€Å"WITH happened to good quality food†¦ â€Å") to those underwhelming with the quality based on price (â€Å"Decent seaf ood but overpriced†, â€Å"Food was fine, but the prices are nuts†), the latter being the most common. Guest comments also suggest that food quality may trump other aspects of the experience in terms of value: â€Å"Great service! Food was okay†¦ Wasn’t worth the $30†, and â€Å"The waiter was nice though†¦ But that still doesn’t make up for the lack in quality. † Combined with the results from the content analysis, it appears that food quality is the most critical factor for these viewers, and by extension Pederasty’s guests. 6. 4. Identifying an Aspect for Improvement As mentioned previously, recommendations matter more than ratings, and recommendations are based on perceptions of value. Positive recommendations tend to be based on positive perception of food quality, however, these recommendations also tend to mention high prices. High prices are why many consumers report visiting upscale casual restaurants less frequently (Techno, Inc. , 2013), and our observations from online reviews confirm price can impact guests’ stated behavioral intention. Based on this, it seems reasonable to assume that lowering prices while maintaining food quality, should lead to improved behavioral intentions and more positive recommendations. But before we explore the validity of that idea, we need to determine if there is any factor that can be changed to allow such a strategy to be entertained. Once again, we turned to the customer reviews, this time examining portion size. 6. 5. Portion Size Pappas Restaurants are known for large portions. Like food quality, it is factor into which money is invested in order to â€Å"wow† the guest. As we saw in the customer views, being â€Å"wowed† is often the line between finding value in the experience and feeling that it is overpriced. Portion size logically contributes to price; therefore we pulled quotes and analyzed them as we did with value-based quotes above. We aimed to determine if the investment in portion size was having the desired effect on guests. Not surprisingly, the reviewers found the portion size to be large: â€Å"the portions were generous†¦ â€Å", â€Å"Huge portions†, and â€Å"†¦ The baked potato is HUGE†¦ â€Å". 30% of the quotes expressed delight. These referred to portions as â€Å"ample† and â€Å"filling†, or mentioned aging a to-go box home (â€Å"l was very happy to have leftovers from this meal! ). 22% were factual statements such as, â€Å"The portion sizes are big like most restaurants in the states†¦ â€Å", or â€Å"l forgot how big the portions are†¦ â€Å". 48%, while not explicitly negative, did express that portion sizes may be overly large. Quotes like, â€Å"†¦ It was Just too big for me to finish! † suggest that portions are too large for guests to finish. One guest felt that they had â€Å"made the mistake of ordering a side salad†, because he was too full to finish his entree. In addition, guests may be lining like they are wasting food they wished to enjoy, a feeling that may be exacerbated by price (â€Å"l felt bad leaving half my lobster on my plate because I was full and it was a bit pricey’). Most of the quotes that fell in this category, and 39% of the overall portion size quotes, dealt with guest behaviors based on large portion have developed ordering behaviors to limit portion size, for example, â€Å"The portions are large, so I always split†, â€Å"l usually have to order a lunch size†, and â€Å"My son and I split a salad and catfish/shrimp plate. Perhaps the most important quote in this roof comes from a review posted on Yelp. Com on January fifteenth, 2013. The guest begins the review by giving background on their relationship with Pudenda: â€Å"As a Native Historian, I’ve enjoyed one of my family’s great traditions of dining at Pudenda to celebrate most any occasion – birthdays, graduations, or Just- because-it’s-the-weekends. Over the years, we’ve gathered at various locations throughout the greater Houston area, but as of lately, this is my frequent location of this chain. They go on to give a glowing paragraph long endorsement of food quality, which concludes with this quote: FYI Just about every dish here has monstrous portions so you wouldn’t be the first to share a plate with someone. † The person writing this article is a champion for the Pudenda brand. She feels a connection to the concept and actively recommends visiting the restaurant. This is meaningful, because in online reviews, recommendations relate to sales, and her recommendation is to share. Guests did not link portion size to value as strongly as they did with food quality. Additionally, explicit complaints portion size was rare, including comments about portion size being too small. This suggests guests view portion size as an enhancement rather than a critical factor when calculating value. While it may be possible that large portion size contributes more greatly to guest delight and it is obscured by reviewers’ choice of language, the fact that almost half of the quotes point to portions being oversized, suggests that they are, in actuality, oversized. . Conclusion 7. 1 . Recommendation Based on our analysis of customer reviews, the investment placed into providing large portions exceeds its relative importance to guests. Guests also report that while they have a largely favorable perception of value, they still find prices to be gig. Food quality was identified by guests as the most critical factor effecting impressions of value. Figure 7 shows their relative positions in terms of investment from the company versus relative importance to guests. By decreasing portion size and lowering prices proportionally, both can be set at new, more appropriate levels without changing food quality. Figure 7 Performance vs.. Importance to Customers Similar strategies have been shown to increase traffic, revenue, and APP. In 2007, they termed â€Å"right portion, right price. † Within a year, these new items were contributing up to 15% of sales per month. They also saw an increase in APP, as more customers were ordering appetizer and deserts. Their success led to the adoption of similar strategies by others, such as Cheesecake Factory and Minim’s Cafe © Innings, 2008). Research from outside the industry suggests that Pudenda may benefit more greatly than others by the implementation of such a strategy. Studies have shown that it grocery stores, high quality food brands gain the most running a promotion with lower prices (Sparkman, 1997). Pudenda has a reputation for high food quality, and it is possible that consumers would view lower prices as a reason to read up, similarly to how they have been shown to behave in grocery stores. . 2. Implementation As large portions are a part of the Pappas brand, and some guests continue to express delight, we suggest maintaining the current menu. New menu items featuring reduced portion and price should be developed and offered separately as has been done by other companies. Tests should be conducted at select locations, and sales can determine their success. In addition, tic kets with the new items should be analyzed to determine if they are contributing to an increase APP. Customer dieback should also be utilized, and can be used to refine the new menu. How to cite Business proposal for Papadeaux, Papers

The Death of a Hero Review Essay Example

The Death of a Hero Review Paper Essay on The Death of a Hero I can not say that the book is bad, that it is not interesting, no. In the novel, is definitely something there, but not for me The essence of the novel is revealed in the very first part, -. But whats there! (in the prologue) on the first page, we learn that the main character was killed. And then we pretty (yes!), And with some irony (sometimes very caustic) tell who and how took the death of Joji Winterbourne, Captain 2roty 9 battalion Fodershirskogo regiment. The mother found out about the death of his son in bed young lover, the father learned of this news on the job (after which he immersed himself in prayer, and he was hit by a car), his wife Elizabeth just startled by the sad news (the frightened artist Swede, who accompanied her home) and his mistress (and girlfriend wife ) Fanny probably not even grieve In essence, this eats s all the characters of the novel. The following sections tell all the vicissitudes of life Winterbourne family, starting with my grandparents; we offer silence and isolation of the thin nature of George, his desire to become an artist; acquaintance with Elizabeth, and their progressive views on life and relationships between the sexes, free relationship of husband and wife sometimes look straight caricature; unwillingness to cause a living creature harm (and especially death) do not interfere with Winterbourne leave to volunteer for the front, even if hes just running problems in the complicated relationship with his wife and Fanny. We will write a custom essay sample on The Death of a Hero Review specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on The Death of a Hero Review specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on The Death of a Hero Review specifically for you FOR ONLY $16.38 $13.9/page Hire Writer The war on the western front, a rather large chunk novel, shown without embellishment, very natural, it seemed to me. Trench life, gas attacks, corpses, cold and lice in dugouts and cellars, shelling, meaningless fights and attacks, fear, blood all that can be in the war, we see through the eyes of a participant. Aldington he was at the front and went through it, he did not have to invent. Just did not have to invent the writer and duplicity, and moral degradation of the society; he denounces jingo, from which there is no sense even in the rear

Free Essays on Pridge And Prejudice Plot Summary

In 1813, Jane Austen published Pride and Prejudice. In this humorous, yet earnest story, Elizabeth Bennet, an unyielding and spirited character, meets a contrasting young man, Mr. Darcy. Darcy is visiting Charles Bingley, the handsome and wealthy bachelor who has recently moved to the Bennet’s hometown of Loungborne. Through the winding, fairytale-like love story of these two retrograde personalities, Elizabeth discovers Mr. Darcy’s haughtiness and imposing character is quite different than expected. His feelings toward Elizabeth evolve from arrogance to love over the course of the page-turning novel. Elizabeth chases her tail searching for truth in this epic love story and her entertaining, estrogen-bubbling family seeks matrimonial pursuits for each daughter. To Mrs. Bennet’s hopeful delight, Mr. Bingley takes a liking to her graceful daughter Jane. But when the Bingleys and Darcy suddenly and without word leave the manor of Netherfield, intending to return to London, Jane’s heart is broken. Wickham, a handsome young soldier, notifies Elizabeth that Mr. Darcy had heartlessly cheated him out of an inheritance. The story continues weaving vignettes of the Bennet girls searching, through their thoughts and their travels, for the reality of the rumors and intentions of the men in their lives. Pride and Prejudice paints a colorful picture of the pursuits of a family seeking wealth and love through marriage for their daughters in the nineteenth century.... Free Essays on Pridge And Prejudice Plot Summary Free Essays on Pridge And Prejudice Plot Summary In 1813, Jane Austen published Pride and Prejudice. In this humorous, yet earnest story, Elizabeth Bennet, an unyielding and spirited character, meets a contrasting young man, Mr. Darcy. Darcy is visiting Charles Bingley, the handsome and wealthy bachelor who has recently moved to the Bennet’s hometown of Loungborne. Through the winding, fairytale-like love story of these two retrograde personalities, Elizabeth discovers Mr. Darcy’s haughtiness and imposing character is quite different than expected. His feelings toward Elizabeth evolve from arrogance to love over the course of the page-turning novel. Elizabeth chases her tail searching for truth in this epic love story and her entertaining, estrogen-bubbling family seeks matrimonial pursuits for each daughter. To Mrs. Bennet’s hopeful delight, Mr. Bingley takes a liking to her graceful daughter Jane. But when the Bingleys and Darcy suddenly and without word leave the manor of Netherfield, intending to return to London, Jane’s heart is broken. Wickham, a handsome young soldier, notifies Elizabeth that Mr. Darcy had heartlessly cheated him out of an inheritance. The story continues weaving vignettes of the Bennet girls searching, through their thoughts and their travels, for the reality of the rumors and intentions of the men in their lives. Pride and Prejudice paints a colorful picture of the pursuits of a family seeking wealth and love through marriage for their daughters in the nineteenth century....

Writers Can Learn from Middlebrow Masters

Writers Can Learn from Middlebrow Masters Writers Can Learn from Middlebrow Masters Writers Can Learn from Middlebrow Masters By Mark Nichol After several years of intending to read through Patrick O’Brian’s Aubrey/Maturin series of seafaring novels, I’ve finally embarked on that voyage, and I’m delighted to note that O’Brian proves that writers can draw lessons in technique from fiction that doesn’t necessarily make it onto too many Great Literature reading lists. O’Brian wrote twenty novels featuring fictional early nineteenth-century Royal Navy officer Jack Aubrey and his friend, naval surgeon Stephen Maturin, over the course of several decades, leaving another one unfinished when he died in 1999. (It was later published in its incomplete form.) After completing the first installment, Master and Commander, I suspect that they’re all ripping good yarns but not (despite some comparisons to the works of Jane Austen and other literary giants) classics for the ages. Yet they’re instructive in how to write and, in one respect, how not to write. First, the bad news: O’Brian, facing the significant challenge of explaining the naval terminology, traditions, and hierarchy of the Napoleonic era to the many readers unfamiliar with such matters, solved it by having various characters explain nautical concepts to Maturin, a landlubber. Unfortunately, though this technique is reasonable in moderation, here it’s employed to extremes. At times, it’s no more subtle than the satirically excessive exposition in the Austin Powers movie series, with the character Basil Exposition laboriously providing background information to the protagonist (and the audience). But the author’s successful avoidance of narrative exposition (that is, other than in dialogue) is related to his great strength: O’Brian rarely employs attribution; the reader usually knows who is talking. But even more remarkable, he rarely has to describe his masterfully well-developed characters. Aubrey and Maturin are an odd couple; the officer is a big and brash yet charismatic leader, while his friend is a quiet, studious surgeon/naturalist/philosopher. The author subtly signals the doctor’s initial unease with shipboard life (he gets in sailors’ way or hits his head on the low beams belowdecks) and his preoccupation with surgical procedures and natural phenomena by indirect reference. Among the best small moments are those in which Maturin tries to engage the practical and intelligent but unschooled Aubrey in intellectual discussion. I did not take advantage of opportunities to work my way through the literary-classics canon during my own schooling, and I am at sea when it comes to lit crit. (If I were asked to analyze the subtext of a cornerstone of the literary tradition, I would probably blithely blink without comprehension much like Aubrey does when confronted with a Latin expression.) But I found myself very much impressed (without being very much distracted) by the mastery with which O’Brian conveys character without describing his characters. I am certain that such lessons in narrative technique can be drawn from many novelists great and small (and in between), and you likely can relate your favorite epiphany of this type. This point only proves that wisdom and inspiration are to be found in unexpected places. Enjoy your pulp fiction, airport novels, beach books, light reading in whatever form your leisure reading takes (including enjoying Great Literature) but be receptive to such insights. Want to improve your English in five minutes a day? Get a subscription and start receiving our writing tips and exercises daily! Keep learning! Browse the Fiction Writing category, check our popular posts, or choose a related post below:10 Grammar Mistakes You Should Avoid55 Boxing IdiomsPassed vs Past

Video Business Case Report on acQuire Technology Solutions - Case 2 Study

Video Business Report on acQuire Technology Solutions - 2 - Case Study Example The paper provides the best alternative that the organization might adopt in order to deal with a situation of weak financial position in future. It is important for the organization to maintain a good customer relationship and upgrade itself with the technological developments taking place in the international market. The most important decision for the concerned organization is that of following a strategy of combining its pricing and marketing strategies in order to improve financial position of the company. The strategy has been recommended with the aim of helping the company to increase its total revenue and also its share in the world market. Introduction The major causes of the financial crisis are manipulations of the financial statements of companies, deficiencies in risk management, high leverage, lack of secured lending in the derivatives market and ineffective management (Bernanke 2010; Gramley 2013). All these led to the development of weak crisis-management capabilities by the companies (Friedman 2011, 98). As a result of the crisis, most of the open economies in the world have been the victims of recession and the companies operating multinationally are struggling to fight the effects of recession. This paper presents a report on a particular issue currently faced by a company named ‘acQuire Technology Solutions’. ... The outcome of this analysis would be useful in developing the decision criteria for the company and identify the alternatives that would be implemented while dealing with the issue currently faced by the company. Issues The world has recently emerged from the turbulent financial crisis and is still fighting to get rid of the after effects of the phenomenon. The concern shown by the CEO of acQuire Technology Solutions, Warren Cook, is that there is distinct possibility of recurrence of the 2008 crisis. This situation would be detrimental for the company and place it in a position of huge loss (Cook 2013a). In this context, the prime issue identified by the company is the development of strong strategy that would help the company deal with the current situation and emerge with success. Causes and importance of the issue The issue identified in the above section is important for acQuire since it is associated with long term sustainability of the company. According to the CEO, the long term performance of the company would be reflected in the way, the management of the organization deals with their responsibility towards their employees and treats their employees at present (Cook 2013b). acQuire Technology Solutions nurtures the objective of providing a long and cherished career to its employees. Maintaining the organization in such a way that it would be able to provide secure career to its staffs is one of the foremost concern for the firm. Decision Criteria Analysis Situational Risk Assessment acQuire Technology Solutions is a privately owned organization and it must make the comparison between risk and reward of any particular decision. The alternatives recommendations that are being

Cash in hand Assignment Example | Topics and Well Written Essays - 250 words

Cash in hand - Assignment Example Mr. Thompson should have minimized his tax liability through legal tax planning whereby the government provides various tools and mechanisms to reduce tax returns such as exemptions, rebates, deductions, and allowances (Gavai 2010). Legal tax planning helps a business to minimize its expenses and thereby reducing its costs of operation and later having long term profits. Therefore, Mr. Thompson should have used other legal means such as low tax rates or other tax benefits that his country offers for investment. The government should ensure that there is tax transparency and ensures that taxes are disclosed to the public. Information sharing and modernization of international tax laws are ways that can reduce tax evasion. Other means of reducing tax evasion include keeping corporate income tax rates low, pursuing international tax information exchange agreements, tightening anti-avoidance rules and also coming up with new tax policies that can help businesses compete (Gavai

Assembly of Functional Cellulolytic Enzymes

Assembly of Functional Cellulolytic Enzymes In the present study, we reported the assembly of functional cellulolytic enzymes using a synthetic, cell-surface engineered yeast consortium. Trichoderma reesei endoglucanase II (EGII) and cellobiohydrolase II (CBHII) and Aspergillus aculeatus ÃŽ ²-glucosidase I (BGLI) were displayed as fusion proteins with the AGA2p C-terminus of a-agglutinin on the cell surface of the diploid yeast strain Saccharomyces. cerevisiae Y5. The immobilization of each enzyme on the cell surface was confirmed by immunofluorescence microscopy. This type of yeast consortium allowed convenient optimization of ethanol production by adjusting the combination ratios of each cell type for inducing synergy in cellulose hydrolysis. Next, the direct ethanol fermentation from steam-exploded corn stover was investigated. The optimized cellulase-displaying consortium produced 20.4 g/l ethanol from 48.4 g cellulose per liter after 72 h in the presence of a small amount of cellulase reagent (0.9 FPU/ml). These findings suggested the feasibility of the cellulase-displaying yeast consortium for simultaneous saccharification and fermentation. Currently, many technological barriers exist with respect to the economical production of ethanol from lignocellulosic biomasses [1]. In the process of hydrolyzing cellulose into soluble sugars, multiple cellulases including endoglucanase (EG), cellobiohydrolase (CBH), and ÃŽ ²-glucosidase (BGL) are required [2]. Consolidated bioprocessing (CBP), which combines enzyme production, hydrolysis, and fermentation in one step, is a promising strategy for effective ethanol production from lignocellulosic materials. Saccharomyces cerevisiae is the traditional microorganism used for ethanol production, but it is unable to utilize cellulosic materials and a saccharification process is required prior to fermentation to produce glucose [3-4]. Numerous attempts have been made to engineer S. cerevisiae strains to express cellulases by cell surface engineering for direct ethanol production from cellulose, and although various bifunctional or trifunctional cellulose-degrading strains have been const ructed, the efficiency of cellulose degradation has not been sufficiently improved [5-9]. It would appear that co-expression of all cellulolytic enzymes in a single cell resulted in relatively low expression levels of cellulases, which may have been due to the heavy metabolic burden and potential jamming of the secretion machinery [6,7,10]. Therefore, in this study, we adapted a new strategy of performing simultaneous saccharification and fermentation with a synthetically engineered yeast consortium having the desired properties of cellulolytic ability and ethanol production to reduce the metabolic burden. The development of a diploid yeast strain is another promising strategy for improving expression levels of heterologous genes and enhancing the fermentation performance of S. cerevisiae. Because diploid strains have better growth ability as well as stress tolerances compared with haploid strains, they are particularly suited for industrial applications. Previously, our group reported on the construction of an à Ã‚ °-agglutinin expression system for genetic immobilization ÃŽ ²-glucosidase I on the cell surface of S. cerevisiae Y5 (Patent No: ZL200810222897.7, CGMCC2660). This diploid robust yeast strain possessed many advantages, such as higher ethanol yield, higher resistance to ethanol, and higher physiological tolerance to inhibitors present in lignocellulosic hydrolysates. Here, we report on our efforts to demonstrate the assembly of functional cellulolytic enzymes using a synthetic yeast consortium. In this study, we demonstrated the feasibility of constructing a novel cell surface engineered diploid yeast consortium for direct ethanol production from phosphoric acid swollen cellulose (PASC) and steam-exploded corn stover (CS), an important step toward direct ethanol production from insoluble cellulosic materials. The strains and plasmids used in this study are summarized in Table S1. Saccharomyces cerevisiae Y5 used for the yeast cell surface display of the cellulolytic enzymes was a newly developed diploid strain in our laboratory. E. coli Top 10 was used as the host strain for recombinant DNA manipulation. T. reesei was purchased from CICC (China Center of Industrial Culture Collection). E. coli transformants were grown in Luria-Bertani medium (1% tryptone, 0.5% yeast extract and 1% NaCl, pH 7.0) supplemented with 100 ug/ml of ampicillin. S. cerevisiae Y5 transformants were selected and maintained on Geneticin plates (1% yeast extract, 2% peptone and 2% glucose supplemented with 600 ug/ml Geneticin) at 30 °C , were induced in YPG (1% yeast extract, 2% peptone, and 2% galactose) at 20 °C. The fermentation medium was composed of 10 g/l yeast extract, 20 g/l polypeptone and 10 g/l PASC as the sole carbon source. The à ¯Ã‚ ¬Ã‚ lamentous fungus T. reesei was cultured in potato dextrose aga r medium (2% potato extract, 2% glucose) at 27 °C. The cDNA was synthesized from mRNA by using the First-Strand cDNA synthesis kit (Fermentas). Unless otherwise indicated, all chemicals, media components and supplements were of analytical grade standard and obtained from Sigma-Aldrich (St. Louis, MO, USA). All restriction enzymes were purchased from New England BioLabs (Ltd. Beijing). Primers used for plasmid construction are provided in Table S2. Plasmid pAGA1 for over-expression of the AGA1 gene and plasmid pBGLI for cell surface display BGLI were constructed previously [11]. Plasmid pEGII for cell surface expression of the EGII (egl2) was constructed as follows. The 1194 bp DNA fragment encoding the egl2 gene without its native secretion signal was amplià ¯Ã‚ ¬Ã‚ ed with the à ¯Ã‚ ¬Ã‚ rst-strand cDNA prepared from T. reesei as the template using primer pairs egl2-For/Rev, this DNA fragment was introduced into the yeast display vector pYD1(Invitrogen) with Kpn I/BamH I. MAT terminator was amplified from pYD1 by using primer pairs MAT-For/Rev and then digested with BamH I/EcoR I to create plasmid pYD1-egl2MAT. The KanR fragment was obtained from plasmid YIP5-KanR by two-step cloning. First, the DNA fragment containing ADH promoter and KanR ORF was amplified from YIP5-KanR by PCR using the KanR-For/Rev primers and inserted into EcoR I/Apa I site of plasmid pYD1-egl2MAT; next, the ADH terminator digested with Bgl II/Nde I was also introduced into pYD1-egl2MAT. The resulting plasmid was named pEGII. For displaying the T. reesei CBHII gene (cbh2) in S. cer evisiae Y5, plasmid pCBHII was created. A 1344 bp gene fragment coding for the mature region of the CBHII was amplià ¯Ã‚ ¬Ã‚ ed using primers cbh2-For/Rev-KT and introduced into plasmid pEGII digested with Kpn I/BamH I for replacing egl2 to form pCBHII (Figure 1). Transformation of S. cerevisiae Y5 was carried out using the lithium acetate method [12]. The plasmid pAGA1 was linearized by Apa I for chromosome integration. The plasmid pYD1 was transformed into S. cerevisiae Y5 as a negative control. S. cerevisiae Y5 clones transformed with different plasmids (strain Y5/pYD1 contained plasmids pAGA1 and pYD1, strain Y5/EGII contained plasmids pAGA1 and pEGII, strain Y5/CBHII contained plasmids pAGA1 and pCBHII) were selected and maintained on Geneticin(G418) plates. Immunofluorescence microscopy was performed as described previously [13]. Immunostaining was performed as follows. Induced recombinant yeast cells expressing cellulases were harvested by centrifugation at 6000 rpm for 5 min and washed with phosphate-buffered saline (PBS). As the primary antibody, mouse anti-Xpress tag antibody (Invitrogen, R910-25) for EGII and CBHII was used at dilution rates of 1:1000. As the second antibody, Fuorescein (FITC)-conjugated goat anti-mouse IgG(H+L) (Jackson, 115-095-003) was used at dilution rate 1:200. Cells and the anti-body were incubated at room temperature. After washing the cell–antibody complex with PBS twice, cellular localizations of the cellulases were observed under a fluorescence microscope. Yeast strains Y5 and Y5/pYD1were used as control. Yeast cells were induced in YPG medium for 48 h at 20 ºC and harvested by centrifugation for 5 min at 6000 rpm, washed with distilled water. BGLI activity of strain Y5/BGLI was measured using à ¯Ã‚ Ã‚ ²-nitrophenyl-ÃŽ ²-D-glucopyranoside as the substrate according to a previously described method [14]. Endoglucanase and cellobiohydrolase activities were determined by hydrolysis of carboxymethyl cellulose (CMC) and phosphoric acid swollen cellulose (PASC), respectively. PASC was prepared from Avicel PH-101 (Fluka Chemie GmbH, Buchs, Switzerland) as amorphous cellulose. The cell pellet was resuspended in a reaction mixture of 1% CMC or 1% PASC in 50 mM sodium acetate buffer (pH 5.0) with the optical density at 600 nm adjusted to 1.0. After a reaction at 50 ºC for 30 min, the activities were determined by DNS method [15]. One unit of enzyme activity was defined as the amount of enzyme released 1 ÃŽ ¼mol reducing sugar from the substrate per minute. The abilities of the engineered yeast consortium (Y5/EGII + Y5/CBHII + Y5/BGLI) to fermentation ethanol from PASC and steam-exploded corn stover were investigated. The steam-exploded corn stover used in this study was provided by Henan Tian Guan Group Co., Ltd (Nanyang, Henan, China). The raw material was chopped to 2-3 cm size and treated in a steam-exploded vessel at 2.0 MPa for 5 min. The pretreated feedstock was dried at room temperature and directly used as a substrate without washing. The moisture content of the substrate was 8%. The composition of materials was quantitatively analyzed following the NREL Laboratory Analytical Procedure NREL/TP-510-42618 (Structural carbohydrates and lignin) (Sluiter et al., 2008)[16], as shown in Table 3. An enzyme mixture composed of equal amounts of cellulase (Sigma-Aldrich, St. Louis, MO) and ÃŽ ²-glucosidase (Sigma-Aldrich) was used. Yeast cells harboring different surface-display plasmid for EGII, CBHII, or BGLI, were grown in YPD medium a nd then transferred to YPG medium for 48 h at 20 ºC to express cellulase. Cells collected by centrifugation at 5000 rpm for 5 min at 4 ºC, washed with distilled water twice, and mixed in the adjustable ratio to a total initial cell concentration of 30 g/l wet weight to form the functional consortium. Ethanol fermentation proceeded at 30 ºC with 90 rpm in 250 ml Erlenmeyer flasks. 1ml samples of the fermentation broth were taken periodically and stored at -4 ºC until they were analyzed for sugar and ethanol content. The total sugar was determined by the phenol-sulfuric acid method [17]. Glucose was measured by HPLC (model 1260, Agilent Technologies) equipped with a Hi-Plex H column 300 mm Ãâ€" 7.7 mm) and a refractory index (RI) detector. Samples were run at a temperature of 60 ºC and a mobile phase of 5 mM sulfuric acid at a flow rate of 0.6 ml/min. Ethanol analysis was carried out using GC (model 7890A, Agilent Technologies) equipped with a flame ionization detector and a HJ-PEG column. Samples were run under the following conditions: column oven at 120 ºC, front injection port at 200 ºC, with N2 as the carrier gas at a flow rate of 4 ml/min. The expression plasmids pEGII and pCBHII (Fig. 1) were transformed into the yeast S.cerevisiae Y5 strains, respectively. All of recombinant yeast strains had a pAGA1 plasmid for integrating AGA1 into the chromosome, and the resultant transformants were designated strains Y5/EGII and Y5/CBHII (Table S1). Upon galactose induction, the proteins were expected to be secreted and interact with the Aga1p and Aga2p anchor system by using the glycosylphosphatidylinositol (GPI) anchor linked to the cell surface. To confirm displaying of EGII and CBHII on the yeast cell surface, immunofluorescence labeling of the cells was carried out using mouse anti-Xpress IgG antibody as the primary antibody. The green fluorescence of Fuorescein (FITC)-conjugated goat anti-mouse IgG was observed for strains Y5/EGII and Y5/CBHII (Fig. 2), indicating that EGII and CBHII were displayed on the cell surface, respectively. The cells harboring the control plasmids were hardly labeled with mouse anti-Xpress IgG(Fig. 2). These results suggested that two types of cellulase were successfully expressed on the cell surface of S. cerevisiae Y5 strain. As shown in Table 1, EGII, CBHII and BGLI activities were detected in the pellet fraction of strain Y5/EGII, Y5/CBHII and Y5/BGLI, respectively. The strain Y5/CBHII and strain Y5/EGII showed moderate CBHII and EGII activity (1.14 U/OD600 and 1.27 U/OD600, respectively). The BGLI activity of strain Y5/BGLI cells was relatively low, which was only 0.72 U/OD600. No enzyme activity was detected in the culture supernatant (data not shown), and the control strain without displayed enzymes exhibited less than 0.1 U/OD600 of enzyme activity. These results clearly indicated that active enzymes were displayed on the cell surface without leakage into the culture medium. Ethanol fermentation from 10 g amorphous cellulose per liter was performed using a cell combination system consisted of three cellulase-displaying yeast populations. Cells displaying EGII, CBHII and BGLI were mixed in various ratios and the produced ethanol from PASC were measured. S.cerevisiae Y5 without displayed enzymes was the control strain. A mixture of cells with EGII: CBHII: BGLI ratio of 2:1:1 produced the highest amount of ethanol (1.76 g/l) after 84 h; the yield (in grams of ethanol produced per gram of consumed reducing sugar) was 0.42 g/g (Fig. 3). A mixture of cells composed of an equal amount of each cell type produced 0.68 g/l ethanol after 84 h (Figure 3), indicating about 1.6-fold improvement of ethanol production by optimizing the cell ratio. However, a large portion of the substrate (the amount of residual sugar after 84 h hydrolysis of 10 g/l PASC was 5.5 g/l, and the sugar consumption rate was 43.3%) remained after 96 h without being hydrolyzed because the cellu lase activities displaying on cell surface were not enough for complete cellulose digestion. Simultaneous saccharification and fermentation of steam-exploded corn stover (CS) as a sole carbon source was conducted for the cellulase-displaying yeast consortium of the optimized ratio 2:1:1 in the presence of commercial cellulase (Sigma-Aldrich, St. Louis, MO) with different enzyme loadings (0, 0.3, 0.6, 0.9, 1.2, 1.5 FPU/ml). A mixture of cells was incubated in 100 ml of YP medium (20 g/l peptone, 10 g/l yeast extract) for 1 h to remove residual carbon source, and then resuspended in YP-CS medium (YP medium containing 100 g/l steam-exploded corn stover, corresponding to 48.4g cellulose per liter). As shown in Fig. 4, in the presence of 0, 0.3, 0.6, 0.9, 1.2 and 1.5 FPU/ml cellulase, 34.49, 18.71, 7.03, 2.11, 1.98, and 1.23 g/l of residual cellulose remained after 84h, respectively. Addition of 0.9 FPU/ml cellulase enabled utilization of 92.3% of the initial cellulose (Figure 4). The cellulose hydrolyzed by cellulase-displaying yeast consortium with an additional 0.9 FPU/ml cellulase was nearly the same as that by control strain S.cerevisiae Y5 with an additional 1.5 FPU/ml. These results indicate that cellulases displayed on the yeast cell surface improve hydrolysis of cellulose, although their activities were lower than commercial enzymes. Furthermore, using the optimized cell combination system, the relationship between the amount of added cellulase and final ethanol concentration was investigated. As shown in Fig. 5, in the presence of 0.9 FPU/ml cellulase, the cellulase-displaying consortium produced 20.4 g/l ethanol after 72 h, which was similar to the value (20.9 g/l) obtained by control strain in the presence of 1.5 FPU/ml cellulase (Table 2). Notably, as the ethanol yield reached 86% of the theoretical yield with 0.9 FPU/ml cellulase, the cell-surface engineered system enabled a reduction in the amount of added commercial cellulase. Hydrolysis of crystalline cellulose to glucose requires the sequential reactions of three groups of cellulases: endoglucanase, cellobiohydrolase, and ÃŽ ²-glucosidase. CBP is a one-step process where all steps occur in a single reactor and a single microorganism or microbial consortium converts pretreated biomass to ethanol with no additional commercial enzymes. The key challenge of CBP lies in choosing the optimal host to directly convert lignocellulosic materials to ethanol. In recent years, several researchers have been engaged in co-displaying multiple cellulases in a single cell for direct conversion of cellulose to ethanol [18-21]. However, the enzyme activity can be limited because of the metabolic burden [22]. Furthermore, it is difficult to control the surface expression level of each enzyme for optimal ethanol fermentation. Apiwatanapiwat et al., constructed the engineered yeast strain NBRC-5Es that co-displayed two types of amylolytic enzymes, two types of cellulolytic enz ymes (T. reesei EGII and CBHII), and A. aculeatus BGLI on the cell surface. The NBRC-5Es strain produced 1.04 g/l ethanol from 8.44 g/l of the acid-treated Avicel after 48 h of fermentation and resulted in a large portion of the substrate remaining without being hydrolyzed by the enzymes. In this study, instead of co-displaying all the enzymes in one cell, we developed a cellulase-displaying yeast consortium consisting of three types of yeast cells, each displaying different cellulases. This method allows for convenient optimization of ethanol production by adjusting the combination ratio of each cell type for inducing a synergy in cellulose hydrolysis. Diploidization is also a promising strategy for enhancing the fermentation ability of S. cerevisiae. Because polyploid yeast strains, including diploid strains, have higher cell growth rates, cell yields, and tolerances to various stresses compared with haploid strains, they are particularly suited for industrial application. Therefore, to generate an efficient â€Å"whole-cell biocatalyst† yeast strain related to cellulosic ethanol production, we selected S. cerevisiae Y5, a robust diploid strain, as the host cell based on its fermentation and inhibitor tolerance properties [23-24]. We first explored the possibility of ethanol fermentation from PASC by using the surface-immobilized yeast consortium (Y5/EGII+Y5/CBHII+Y5/BGLI). A mixture of cells at the optimized EGII: CBHII: BGLI ratio of 2:1:1 produced 1.6-fold more ethanol (1.76 g/l) than cells composed of an equal amount of each cell type. Next, the fermentation performance of yeast consortium using steam-exploded CS as the sole carbon source was further investigated. The optimized cellulase-displaying consortium produced 20.4 g/l ethanol from 48.4 g cellulose per liter after 72 h in the presence of a small amount of cellulase reagent (0.9 FPU/ml), suggesting the feasibility of the cellulase-displaying yeast consortium for simultaneous saccharification and fermentation. Although several studies have been carried out on establishing a cell-displaying yeast consortium [25-27], few reports of direct ethanol fermentation from pretreated lignocellulosic material have been published. The combined cell system describ ed here could become the basis for the eventual direct ethanol production from insoluble cellulosic materials.

Anth. 3 Types of Rewards

There are three types of rewards that may or may not be equally distributed within a society. These rewards include wealth, power and prestige. Furthermore, there are three basic types of societies identified by Morton Fried in which the equal or non-equal distribution of these rewards may occur. These societies include that of an egalitarian society, a ranked society, and a stratified society. Egalitarian societies do not rely on wealth or power. Instead, people in this type of society do gain prestige through age, valuable skills, and an attractive personality. Everything is shared equally within this society and there is no reason for wealth because they tend to be a mobile type of society such as the hunter gatherers, and they are not able to bring many possessions with them. Therefore, there is no accumulation of wealth among the people of this society. The ! Kung are an example of a egalitarian society. They have little possessions, which usually denotes wealth in Western culture. They are an extremely mobile people who travel to find food. Therefore, they have no need for possessions because they are not able to bring them with them. They also work on a system of reciprocal sharing. Therefore, this prevents people from gathering wealth or power over others. As a result of being foraging people they have the option of leaving if one person within the band tries to take power over others. In ranked societies, there are a limited number of social positions which grant authority over others in the society. These positions are mostly always gained through heredity. Power and prestige are given to those in high social rank, which usually falls upon the eldest in the lineage. Wealth is usually distributed among the society equally through redistribution. The Tikopia society is an example of a ranked society. The 1200 people of the island were divided into 4 patriclans and each patriclan had its own chief. There are also clan chiefs who have the most authority over others. However, they did not have great power over others within the society because they believed that each had rights to the land and ocean resources within the clan. They were honored but their wealth and power was not great, seeing as they used the system of redistribution. In stratified societies, the rewards of wealth, power, or prestige are not equally distributed within the society. There are two distinguishing characteristics in stratified societies. The first characteristic being inequalities between strata in access to rewards such as wealth power and prestige because they may be obtained through heredity. Secondly, there may be unequal access to resources. There are two types of stratified systems in the world today. The first of these is the caste system. The course text defines a caste system as a â€Å"stratification system in which membership is a stratum is in theory hereditary, strata are endogamous, and contact or relationships among members of different strata are governed by explicit laws, norms, or prohibition. A widely used example of a caste system is in place today in India. Those who are born into the highest caste can look forward to a very promising future filled with wealth and prestige. Those born into the lowest caste have a life of hard labor and no chance of advancement. There are strict laws to enforce marriage between people of differ ent caste, and even social relationships among people of different castes. The second type of social system is known as a class system. A class system is defined in the text as, â€Å"a system in which membership in a stratum can theoretically be altered and intermarriage between strata is allowed. An example of a class system would be that of our own western culture. The easiest way to determine class for our culture is through wealth. The more wealth you have the higher your class, but it differs from that of the caste system because you are able to have social relations with people outside of your own class and you are able to move up in class. Even if born into a â€Å"lower† class you are able to gain access to schools, have access to resources, and are able to marry outside of you

Background of Human Resource Management - 1652 Words

Background of HRM Early studies on human resource management can be traced under the field of the studies of personnel management (Scott, 1915; Asher, 1972; Campbell et al., 1970). However a shift from personnel management to HRM occurred in the early 1980’s. Some authors (Storey, 1994; Torrington et al., 2008) argue that human resource management has two meanings. According to one of them, human resource management covers the same activities that personnel management used to before the shift in the 1980’s. Following another meaning however, personnel management and human resource management differ. Legge (1995) argues that the difference between the two is very thin and is based on the way people are treated, as the†¦show more content†¦incentive pay, pay for performance vs. seniority). †¢ The extents to which reward systems are linked to strategic plans and encourage employees to work toward accomplishing business needs and meeting customer requirements. †¢ The extent to which rewards are based on individual vs. group or corporate performance. ââ€" ª Structure of non-financial rewards (e.g. recognition programs, titles, informal status symbols). Communications and Public Relations †¢ Types of information presented to employees, manner of presentation (e.g. confidential vs. public) †¢ Types of communication channels; dissemination of information inside and outside the organization; opinion of surveys; open door policies. †¢ Design of communication programs (e.g. public meetings, management forums for discussion, videos, written communications, bulletins). ⠝â€" Motivation The term Motivation is divide form word ‘MOTIVES’. Motives are those fore within an individual that compel him to act or not to act in certain ways. So MOTIVATION way be defined as a process of stimulating sub- ordinate to work hard with confidence towards the attainment of organizational goals. In order to motivate a person it is necessary to satisfy his needs, needs may be classified as follows: [pic] ⠝â€" Maintenance: It means arranging for the necessary facilities for the employees. 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Yet, at the same time defining human resource mana gement was elusive with the inability â€Å"in identifying its boundaries, or in distinguishing itRead MoreCase Study : Maple Leaf Shoes Ltd982 Words   |  4 Pageswould ask. During his interview with the president and major shareholder, there were many interruptions such as the phone calls. Using face to face interview process Lance got to learn not only about the way the employees think about the company management but also the non-verbal cues gestures and attitudes of the employees. Wolff (2008) asserts that â€Å"the key to solving underperformance is communication, communication, communication – coupled with clarity about expectations and objectives, early intervention

A Lifestyle Of Learning Essay - 977 Words

A Lifestyle of Learning â€Å"‘ Libby, who was the first president of the United States?’ ‘George Washington!’ ‘Who was the first man to walk on the moon?’ ‘Neil Armstrong!’ ‘How do you spell your name?’ ‘L I B B Y!’† This was a conversation my father and I had often, when I was three and four years old. From a young age, I loved to learn. Facts, Bible verse, spelling, and math; at three years old, I would memorize just about anything. I loved knowledge and the way it empowered me. As a young child, knowledge gained me coveted praise and attention from those older than me, and in elementary and junior high school, knowledge gained me respect from my peers. Now, as an adult, knowledge unlocks whole new worlds, as I explore different ways of thinking, skills and people groups. Since I was three years old, I have lived a lifestyle of learning. Though my mom taught me how to read and w rite, at home, kindergarten was the first experience I had in a formal classroom. 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